Transcription profiling by array of rat ventricular myocytes cultured with recombinant human interleukin 6

ABSTRACT: Ventricular myocytes were isolated from adult male Sprague-Dawley rats (250-350g) by collagenase digestion. Cells were cultured with recombinant human interleukin 6 for six hours prior to isolation total RNA by RNeasy mini kit (Qiagen). RNA was amplified and labelled using the Illumina TotalPrep RNA Amplification kit (Applied Biosystems) and hybridised to RatRef-12 Expression BeadChip (Illumina) according to manufacturer's instructions.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Nazima Pathan 

PROVIDER: E-MTAB-344 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress