ChIP-Seq of human MCF-7 cells with ER-alpha and ER-beta anitbodies following estrogen treatment

ABSTRACT: We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP-ER-beta cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer. (Note: Raw data files not provided by submitter)

INSTRUMENT(S): Solexa 2G Genome Analyzer

ORGANISM(S): Homo sapiens

SUBMITTER: Alessandro Weisz 

PROVIDER: E-MTAB-345 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress