Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

13 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional profiling by mRNA microarray of pancreas-infiltrating T lymphocytes of NOD mice

ABSTRACT: Whole mouse genome microarrays from Agilent were used to determine expression profile of pancreas-infiltrating T CD3+ and total T lymphocytes of NOD mice.

INSTRUMENT(S): Agilent G2565AA and G2565BA Microarray Scanner

ORGANISM(S): Mus musculus

SUBMITTER: Geraldo Passos

PROVIDER: E-MTAB-3555 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcriptional profiling by microarray of microRNAs of pancreas-infiltrating T lymphocytes from NOD mice

Project description:The microRNA oligo microarrays from Agilent were used to determine miRNA expression profile of pancreas-infiltrating T CD3+ and total T lymphocytes of NOD mice.

2015-05-29 | E-MTAB-3556 | biostudies-arrayexpress

The effect of the autoimmune regulator (Aire) gene on the transcriptional expression profile of 3.10 mTEC cell line.

Project description:Medullary thymic epithelial cells (mTECs) contribute to self-tolerance through the ectopic expression of peripheral tissue antigens (PTAs) in the thymus. PTA expression in mTECs is largely dependent on the autoimmune regulator (Aire) gene. Here we used a Mus musculus mTEC cell line (3.10 mTEC line, which constitutively express Aire in culture) to knockdown Aire gene by means of siRNA transfection. Aire knockdown was confirmed by means of qRT-PCR and RNA-FISH (for Aire mRNA levels), immunofluorescence and western blot (for AIRE protein levels).The Agilent oligo microarrays were used to determine the large scale transcriptional expression profiles of control or Aire-knockdown 3.10 mTECs.

2015-12-31 | E-MTAB-3879 | biostudies-arrayexpress

The effect of Dicer knockdown on the transcriptional expression profile of 3.10 mTEC cell line.

Project description:Dicer, also known as endoribonuclease Dicer or helicase with RNase motif, cleaves double-stranded RNA (dsRNA) and pre-microRNA (pre-miRNA) into short mature double-stranded RNA fragments called small interfering RNA and microRNA, respectively. Medullary thymic epithelial cells (mTECs) express close to 90% of the protein-coding genome, besides they play an important role to self-tolerance through the ectopic expression of peripheral tissue antigens (PTAs) in the thymus. MicroRNAs are potent post-transcriptional regulators in developmental switches, lineage commitment and gene expression regulation, but their role in representation of the immunological self in mature mTECs is not fully understood. So we used a Mus musculus mTEC cell line (mTEC 3.10) to knockdown Dicer transcript by means of siRNA transfection and then observe the effect of Dicer knockdown in the transcriptional profile of messenger RNAs (mRNAs) by means of oligo microarray hybridization. The Agilent oligo microarrays were used to determine the large scale mRNA transcriptional profiles of control or Dicer-knockdown 3.10 mTECs.

2016-06-27 | E-MTAB-4628 | biostudies-arrayexpress

Post-transcriptional control of Aire gene by the microRNA 155

Project description:In this study, we transfected murine (Mus musculus) medullary thymic epithelial cell line (mTEC 3.10 cell line) with miR155 mimic to assess a possible post-transcriptional control of the miRNA over the Aire gene. Then we analyzed the differential transcriptional profile of this cell line, by means of Agilent oligo microarray hybridization, comparing Autoimmune regulator (Aire) wild-type cells vs cells transfected with miR155 mimic. The comparative transcriptional expression signatures allowed us to find those differentially expressed mRNAs between the samples tested.

2022-10-01 | E-MTAB-7709 | biostudies-arrayexpress

The role of Autoimmune regulator (Aire) gene in the control of adhesion between medullary thymic epithelial cells and thymocytes

Project description:The crosstalk between thymocytes and thymic epithelial cells is critical for T cell development and establishment of central tolerance. Although the role of Autoimmune Regulator (Aire) gene in the induction of central tolerance is well known, the precise cellular and molecular mechanisms by which Aire controls the ectopic expression of tissue restricted antigens (TRAs) in medullary thymic epithelial cells (mTECS) remain unclear. In this study we performed a functional assay with fresh thymocytes dissociated from a normal mouse thymus co-cultured with a Mus musculus mTEC cell line - named 3.10 mTEC line - , in which we had previously knocked-down Aire gene by means of siRNA transfection. Agilent Mouse Gene Expression microarrays were used to determine the large scale transcriptional expression profiles of control and Aire-knockdown 3.10 mTECs co-cultured with thymocytes.

2016-06-27 | E-MTAB-4595 | biostudies-arrayexpress

Transcriptional profile by mRNA microarrays of cervical thymus, thoracic thymus and parathyroid from Foxn1-GFP; Pth-Cre; R26dTomato transgenic mice

Project description:Whole mouse genome microarrays from Agilent were used to determine expression profile of whole organ cervical thymus, thoracic thymus and parathyroid from Foxn1-GFP; Pth-Cre; R26dTomato transgenic mice.

2015-07-31 | E-MTAB-3751 | biostudies-arrayexpress

Transcriptional profile by microRNA microarrays of cervical thymus and thoracic thymus from Foxn1- GFP; Pth-Cre;R26dTomato transgenic mice

Project description:The microRNA oligo microarrays from Agilent were used to determine the miRNAs expression profile of whole organ cervical and thoracic thymi from Foxn1-GFP; Pth-Cre; R26dTomato transgenic mice.

2015-07-31 | E-MTAB-3752 | biostudies-arrayexpress

The effect of the Autoimmune regulator (Aire) gene on mRNA and lncRNA transcriptional profile of mTEC 3.10 cell line

Project description:In this study, we used the murine (Mus musculus) medullary thymic epithelial cell line (mTEC 3.10 cell line) co-cultured with fresh thymocytes as a functional assay for mTEC-thymocyte adhesion. Then we analyzed the differential transcriptional profile of this cell line, by means of Agilent oligo microarray hybridization, comparing Autoimmune regulator (Aire) wild-type cells vs Crispr-Cas9-induced Aire KO cells. The comparative transcriptional expression signatures allowed us to find those differentially expressed mRNAs or lncRNAs between the samples tested.

2021-12-31 | E-MTAB-6541 | biostudies-arrayexpress

Identification of differentially expressed genes in human alveolar bone cells cultured on different titanium surfaces.

Project description:To evaluate mRNA expression profile (transcriptome), we identified mRNAs of osteoblastic cells from human alveolar crest culture in contact with different titanium surfaces: control (polished), nanotextured (polished titanium discs, etching at 25oC with 50% H2SO4conc and 50% H2O2aq) , nano+submicrotextured (polished titanium discs, etching at 50oC with 50% H2SO4conc and 50% H2O2aq) and rough microtexture (polished titanium discs, etching at 50oC with 100% H2O2aq). The osteoblastic cells were cultured in the alpha-minimum essential medium, supplemented with fetal bovine serum, gentamicin, fungizone, dexamethasone, ascorbic acid and β-glycerophosphate. The mRNA expression profiling was analyzed through hybridizations with whole-human genome Agilent microarray (4x44K format).

2014-11-14 | E-MTAB-3091 | biostudies-arrayexpress

Identification of differentially expressed microRNA in human alveolar bone cells cultured on different titanium surfaces.

Project description:To evaluate miRNA expression profile (miRNOME), we identified microRNAs of osteoblastic cells from human alveolar crest culture in contact with different titanium surfaces: control (polished), nanotextured (polished titanium discs, etching at 25°C with 50% H2SO4conc and 50% H2O2aq) , nano+submicrotextured (polished titanium discs, etching at 50°C with 50% H2SO4conc and 50% H2O2aq) and rough microtexture (polished titanium discs, etching at 50°C with 100% H2O2aq). The osteoblastic cells were cultured in the alpha-minimum essential medium, supplemented with fetal bovine serum, gentamicin, fungizone, dexamethasone, ascorbic acid and β-glycerophosphate. The microRNA expression profiling was analyzed through hybridizations with Agilent miRNA-microarray (8x15K format).

2014-12-12 | E-MTAB-3093 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data