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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Exogenous BRCT-GST of PARP1 bind to wild-type p53. p53 was overxpressed after UV treatment in U2OS.

ABSTRACT: Exogenous BRCT-GST in U2OS bind to wild-type p53 in a phosphorylation-dependent manner, after UV treatment. BRCT-GST fusion protected cells from apoptotic cell death.

ORGANISM(S): Homo sapiens

SUBMITTER: Marco Galasso

PROVIDER: E-MTAB-3619 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Exogenous BRCT-GST of PARP1 bind to wild-type p53. p53 was overexpressed after UV treatment in U2OS.

Project description:Exogenous BRCT-GST in U2OS bind to wild-type p53 in a phosphorylation-dependent manner, after UV treatment. BRCT-GST fusion protected cells from apoptotic cell death and impact of miRNAs responsive to p53 activation.

2024-03-02 | E-MTAB-3628 | biostudies-arrayexpress

RNA sequencing analysis of HEK293T cells infected with a lentivirus coding the BRCT domain from Leishmania major Pes protein

Project description:Given the prevalence of leishmaniasis and cancer, the co-existence of these two diseases may be merely coincidental. However, a number of epidemiological studies suggest that an association between these two entities does exist. Mammalian PES protein has been reported to be involved in different cellular processes such as cell cycle regulation. Its BRCT domain has been identified as a key factor in DNA damage-responsive checkpoints. We aimed to elucidate the hypothetical oncogenic character of this BRCT domain from Leishmania major PES protein (LmPES) on the host cells. For this purpose, we generated a lentivirus carrying this BRCT domain sequence (lentiBRCT) and a lentivirus expressing the lucifarese protein (lentiLuc), as control. Then, HEK293T mammal cells were infected with these lentivirus. Gene expression profiling analysis revealed that BRCT domain from LmPES protein altered the expression of proliferation, survival and chemoresistance related genes. In addition, RNA sequencing study showed a down-regulation of mitochondrial genes in the BRCT domain expressing cells triggering a mitochondrial dysfunction. Altogether, our results reinforce the idea that in eukaryotes, horizontal gene transfer may be also achieved by parasitism like Leishmania infection driving therefore to some crucial biological changes such as proliferation and drug resistance.

2022-11-16 | GSE160853 | GEO

CINC-2 and miR-199a-5p in exosomes secreted by transplanted Thy1+ cells activate hepatocytic progenitor cell growth in rat liver regeneration

Project description:Small hepatocyte-like progenitor cells (SHPCs) are hepatocytic progenitor cells that transiently form clusters in rat livers treated with retrorsine and with 70% partial hepatectomy (PH). We previously reported that transplantation of Thy1+ cells derived from D-galactosamine-treated livers promotes SHPC expansion, resulting in the acceleration of liver regeneration. Extracellular vesicles (EVs) produced by Thy1+ cells act on sinusoidal endothelial cells (SECs) and Kupffer cells to secrete IL17B and IL25, respectively, resulting in SHPC activation through IL17 receptor B (RB) signaling. Our aim is to identify factors in Thy1-EVs that activate IL17RB signaling. Thy1+ cells isolated from rats with D-galactosamine-induced liver injury were cultured for one week. Although some liver stem/progenitor cells proliferated into colonies, others maintained as mesenchymal cells (MCs). Thy1-MCs or Thy1-liver stem/progenitor cells were transplanted into retrorsine/PHtreated livers to examine their effects on SHPCs. SHs isolated from adult rat livers were used to validate factors regulating growth induction. The number and size of SHPCs remarkably increased in livers transplanted with Thy1-MCs. Comprehensive analysis of Thy1-MC-EVs revealed that miR-199a-5p, CINC-2, and MCP-1 are candidates for stimulating SHPC growth. Administration of the miR-199a-5p mimic, and not CINC-2, promoted SH growth. SECs treated with CINC-2 induced IL17b expression and their conditioned medium promoted SH growth. Thy1-MC transplantation may accelerate liver regeneration due to SHPCs expansion, which is stimulated by CINC-2/IL17RB signaling and miR-199a-5p.

2023-04-07 | PXD039384 | Pride

Saccharomyces cerevisiae

Project description:Expression profile in Overexpression of GST,GST-Med3

| PRJNA274807 | ENA

Transcription profiling of synthetic allopolyploid mature flower comparison in Senecio cambrensis

Project description: Not available

2006-09-19 | E-MAXD-5 | biostudies-arrayexpress

Zea mays B73 stress treatments on UA 70mer oligo slides (Maize oligo array version 1.3 array A and B)

Project description:Maize seedling transcriptome responses to six abiotic perturbations (heat, cold, darkness, desiccation, salt, UV-B) and 2 controls were analyzed Keywords: comparitive transcriptome profiling Tissues were grown at Stanford University during the summer of 2005. RNA was extracted and purified, cDNA generated, and amplified cRNA was labeled with Cy3 or Cy5 dyes from 4 biological replicates of each treatment. Each replicate was composed of a pool of 10 similarly treated whole seedlings (with the endosperm and kernel removed). Two types of biological controls were employed: 1) seedlings germinated under constant 26ÂºC temperature, with 16 h light and adequate water and 2) field grown juvenile leaf samples Field seedlings emerged on day 6 or 7.

2008-05-31 | E-GEOD-9453 | biostudies-arrayexpress

DNSN-1 recruits GINS for CMG helicase assembly during DNA replication initiation in C. elegans

Project description:Assembly of the DNA helicase known as CMG (CDC45-MCM-GINS) is the key regulated step during DNA replication initiation in eukaryotes. Using the Caenorhabditis elegans embryo as a model system, we identify a new CMG assembly factor called DNSN-1, which associates with the BRCT-domain protein MUS-101. We show that DNSN-1 is required to recruit the GINS complex to chromatin and find that DNSN-1 positions GINS on the MCM-2-7 helicase motor, by direct binding of DNSN-1 to GINS and MCM-3, on interfaces that are important for initiation and essential for viability.

2023-10-24 | PXD044240 | Pride

Sir4 H-BRCT domain interacts with multiple phospho-proteins to regulate heterochromatic repression

Project description:In Saccharomyces cerevisiae, the Silent Information Regulator (SIR) proteins Sir2/3/4 form a complex suppressing transcription of genes at subtelomeric regions and the homothallic mating type (HM) loci. Here we identify a non-canonical BRCA1 C-terminal domain (H-BRCT) in Sir4 which is responsible for tethering telomeres to the nuclear periphery. We show that Sir4 H-BRCT and the closely related Dbf4 H-BRCT serve as selective phospho-epitope recognition domains that bind to a variety of phosphorylated target peptides. We present detailed structural information about the binding mode of established Sir4 interactors (Esc1, Ty5, Ubp10) and identify several novel interactors of Sir4 H-BRCT, including the E3 ubiquitin ligase Tom1. Based on these findings, we propose a phospho-peptide consensus motif for interaction with Sir4 H-BRCT and Dbf4 H-BRCT. Ablation of the Sir4 H-BRCT phospho-peptide interaction disrupts SIR-mediated repression and perinuclear localization. In conclusion, the Sir4 H-BRCT domain serves as a hub for recruitment of phosphorylated target proteins to heterochromatin to properly regulate silencing and nuclear order.

2019-09-13 | PXD014119 | Pride

Acclimation of photosynthesis to changes in the environment results decreases oxidative stress in Arabidopsis thaliana

Project description:Dynamic acclimation of photosynthesis plays an important role in increasing plant fitness under variable light environments. Acclimation is mediated by a glucose-6-phosphate/phosphate translocator, GPT2. This study examined whether plants lacking GPT2, which are defective in acclimation to increases in light, are more susceptible to oxidative stress. To understand this, we used the model plant of Arabidopsis thaliana (accession Wassilewskija-4 (Ws-4)) and compared this to mutants lacking GPT2. Plants were grown at low light (100 μmol m−2 s−1) for 7 weeks. For acclimation experiments, a set of plant from low light was transferred to 400 μmol m−2 s−1 for 7 days. Microarray analysis showed that gpt2 plants showed a greater induction of stress related genes relative to WT.

2021-03-24 | E-MTAB-10282 | biostudies-arrayexpress

Transcription profiling of mouse bFGF-treated JB6 cells and fully tumorigenic JB6 cells from the RT101 cell line - reversible and irreversible anchorage independent growth in skin cells

Project description:We have investigated the regulation of anchorage-independent growth (AIG) by basic fibroblast growth factor (bFGF) and 12-O-tetradecanoyl phorbol-13-acetate (TPA) in JB6 mouse epidermal cells in the context of wound repair versus carcinogenesis responses. bFGF induces an unusually efficient but reversible AIG response, relative to TPA-induced AIG which is irreversible. Distinct global gene expression profiles are associated with anchorage-independent colonies arising from bFGF-stimulated JB6 cells, relative to colonies arising from fully tumorigenic JB6 cells (RT101), including genes exhibiting reciprocal regulation patterns. Thus, while TPA exposure results in commitment to an irreversible and tumorigenic AIG phenotype, the AIG response to bFGF is reversible with essentially complete restoration of normal cell cycle check point control following removal of bFGF from growth medium. These results are consistent with the physiological role of bFGF in promoting wound healing, and suggest that natural mechanisms exist to reverse transformative cellular phenotypes associated with carcinogenesis. Experiment Overall Design: To determine whether there were shared transcriptional effects between reversible and irreversible AIG, we performed global microarray analyses on colonies isolated from soft agar for both bFGF-treated JB6 cells and fully tumorigenic JB6 cells from the RT101 cell line. Gene expression changes associated with colony formation were determined by comparison to respective monolayer control cells.

2009-06-08 | E-GEOD-13979 | biostudies-arrayexpress

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