Regulated endocleavage balances the degradation of nonsense-mediated decay substrates

ABSTRACT: Nonsense-mediated mRNA decay (NMD) controls gene expression by eliminating mRNAs with premature or aberrant translation termination. Degradation of NMD substrates is initiated by the central NMD factor UPF1, which recruits the endonuclease SMG6 and the deadenylation-promoting SMG5/7 complex. Here we map transcriptome-wide sites of SMG6-mediated endocleavage via 3′ fragment capture and degradome sequencing.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Jernej Ule 

PROVIDER: E-MTAB-3625 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress