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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide distribution of histones modifications in mouse ESC Rif1+/+ and Rif1-/-

ABSTRACT: Analyzed histones modifications: H3K9 H3K4 H3K27 H4K20

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Rossana Foti

PROVIDER: E-MTAB-3743 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nuclear Architecture Organized by Rif1 Underpins the Replication-Timing Program.

Foti Rossana R Gnan Stefano S Cornacchia Daniela D Dileep Vishnu V Bulut-Karslioglu Aydan A Diehl Sarah S Buness Andreas A Klein Felix A FA Huber Wolfgang W Johnstone Ewan E Loos Remco R Bertone Paul P Gilbert David M DM Manke Thomas T Jenuwein Thomas T Buonomo Sara C B SC

Molecular cell 20151224 2

DNA replication is temporally and spatially organized in all eukaryotes, yet the molecular control and biological function of the replication-timing program are unclear. Rif1 is required for normal genome-wide regulation of replication timing, but its molecular function is poorly understood. Here we show that in mouse embryonic stem cells, Rif1 coats late-replicating domains and, with Lamin B1, identifies most of the late-replicating genome. Rif1 is an essential determinant of replication timing ...[more]

PMID: 26725008

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