Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

SKI2 mediates degradation of RISC 5â-cleavage fragments and prevents secondary siRNA production from miRNA targets in Arabidopsis

ABSTRACT: A loss-of-function mutant in the SKI2 helicase in Arabidopsis was shown to accumulate 5'-cleavage fragments of several miRNA targets. Small RNAs in ski2 single and ski2/rdr6 double mutants (two biological replicates of each genotype) were next analysed by NGS. The data show that several miRNA targets produce RDR6-dependent siRNAs upon inactivation of SKI2. Files containing these NGS data are deposited here. Seeds were surface sterilized and germinated on MS medium for 2 weeks. The seedlings were grown on soil until flowering stage in a 16h light period. The inflorescences were harvested and used to prepare RNA. Small RNA libraries were prepared using the NEBnext Multiplex small RNA library prep kit for Illumina sequencing.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Shiraz Shah

PROVIDER: E-MTAB-3781 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

SKI2 mediates degradation of RISC 5'-cleavage fragments and prevents secondary siRNA production from miRNA targets in Arabidopsis.

Branscheid Anja A Marchais Antonin A Schott Gregory G Lange Heike H Gagliardi Dominique D Andersen Stig Uggerhøj SU Voinnet Olivier O Brodersen Peter P

Nucleic acids research 20151012 22

Small regulatory RNAs are fundamental in eukaryotic and prokaryotic gene regulation. In plants, an important element of post-transcriptional control is effected by 20-24 nt microRNAs (miRNAs) and short interfering RNAs (siRNAs) bound to the ARGONAUTE1 (AGO1) protein in an RNA induced silencing complex (RISC). AGO1 may cleave target mRNAs with small RNA complementarity, but the fate of the resulting cleavage fragments remains incompletely understood. Here, we show that SKI2, SKI3 and SKI8, subuni ...[more]

PMID: 26464441

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Dataset Information

SKI2 mediates degradation of RISC 5â-cleavage fragments and prevents secondary siRNA production from miRNA targets in Arabidopsis

Publications

SKI2 mediates degradation of RISC 5'-cleavage fragments and prevents secondary siRNA production from miRNA targets in Arabidopsis.

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SKI2 mediates degradation of RISC 5â-cleavage fragments and prevents secondary siRNA production from miRNA targets in Arabidopsis