Protein occupancy of Mycoplasma pneumoniae chromosome
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ABSTRACT: Protein occupancy is a means of identifying all the regions in the genome that are bound to proteins. A simple protocol is used whereby cross-linked chromatin is digested with DNase to remove all non-crosslinked, naked, DNA. To distinguish the regions that are bound by RNA polymerase from the rest, a ChAPseq (chromatin affinity precipitation) with TAP-tagged alpha subunit of RNApol, was performed.
INSTRUMENT(S): Illumina HiSeq 2500, Illumina Genome Analyzer II
ORGANISM(S): Mycoplasma pneumoniae M129
SUBMITTER: Eva Yus
PROVIDER: E-MTAB-3783 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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