Project description:Two lines of Trypanosoma brucei were isolated from cattle in Uganda. The DNA is from parasites that had undergone two rodent passages.
Project description:Fresh T. b. brucei isolates MAK65 and MAK98 (see E-MTAB-9320) were cultured in vitro for various times then the genomic DNA was sequenced.
Project description:Wild type T. brucei bloodstream form were incubated with increasing concentrations of AN7973, a benzoxaborole compound developed by Anacor Pharmaceuticals Inc. against Animal African Trypanosomiasis. Cells had a tendency to lose resistance, only roughly 2x EC50 resistance was obtained. This experiment include the sequencing of wild type cells, intermediate stages (80C and 80D) and final resistant cell lines (80C3005 and 80D2004)
Project description:Human African Trypanosomiasis (HAT) is a disease of major economic importance in Sub-Saharan Africa. In eastern and southern Africa. Here we analysed clinical isolates of T brucei rhodensiense, resistant to suramin by shotgun proteomics . And identified parasite proteins whose expression is associated with resistance to suramin.