Transcription profiling by array of Saccharomyces cerevisiae in response to ethanol

ABSTRACT: The genome-wide transcriptional responses of the wild type laboratory strain, and two deletion strains (pmt7Δ/pmt7Δ and yhl042wΔ/yhl042wΔ) were comparatively investigated in the absence and in the presence of ethanol for two hours. Strains were cultivated in flasks at 30°C and 180 rpm in an orbital shaker till the mid-exponential phase of growth (OD600 of 0.85-0.95). The exponential cultures were then divided into two flasks and cells in one flask were grown without ethanol, whereas cells in the latter flask were treated with ethanol to have 8% (v/v) final ethanol concentration. In order to analyze the transcriptional response of each strain to ethanol, samples were collected 2 h after ethanol treatment from both treated and untreated cultures, immediately frozen in liquid nitrogen and sto

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Ceyda Kasavi 

PROVIDER: E-MTAB-3874 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress