Haploid and diploid Emiliania huxleyi (RCC 1217 and RCC1216) under P starvation
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ABSTRACT: Haploid (1N) and diploid (2N) Emiliania huxley (RCC 1217 and RCC1216) were grown in dilute batch culture and achieved stationary phase. We sampled two time points, at early (T2) and late (T3) stationary phase. Controls are haploid or diploid cells derived from dilute cultures (T1) growing in replete control medium. All samples were hybridized against a common, pooled baseline RNA. All experiments and hybridization were done in independent biological triplicates (A, B and C).
Project description:Haploid (1N) and diploid (2N) cells of Emiliania huxley (RCC 1217/1216) were grown under 50 and 300 µmol photons m-2 s-1. Differential gene expression was assessed between the ploidy stages at same irradiance and between the light treatments of same strains.
Project description:To investigate the effects of ocean acidification (OA) and their energy-dependent modulation in the life-cycle stages of E.huxleyi, diploid and haploid cells were acclimated to present and future CO2 partial pressures (pCO2; 38.5 Pa vs. 101.3 Pa CO2) under low and high light (50 vs. 300 M-5mol photons m-2 s-1). Microarray-based transcriptome profiling was used to screen for cellular processes that underlie the physiological OA-responses observed earlier (Rokitta & Rost 2012)
Project description:Diploid and haploid strains often exhibit different tolerance to variety of stresses. Transcriptome of acclimation to ethanol stress in diploid and haploid strain of Saccharomyces cerevisiae was analyzed. We analyzed transcriptome profiles of diploid and haploid strains in the presence of ethanol.
Project description:Diploid and haploid strains often exhibit different tolerance to variety of stresses. Transcriptome of acclimation to ethanol stress in diploid and haploid strain of Saccharomyces cerevisiae was analyzed. We analyzed transcriptome profiles of diploid and haploid strains in the presence of ethanol. Haploid and diploid strains were cultured in YEPD media with 0%, 3% and 7% ethanol(v/v) in fermentors. The samples were collected at the growth stage for each strain under different conditions.
Project description:We investigated the effects of the ploidy on cellular response in strains carrying various types of gross chromosomal rearrangements. Fourteen mutated strains (6 haploid strains and 8 diploid strains) were compared to their associated parental strain (haploid or diploid parental strain). For each comparison, 2 microarray experiments implying biological replicates were performed.
Project description:gene expression differences were analysed between haploid and diploid ES cells biological triplicates of haploid and diploid ES cell lines were analysed on an Affymetrix GeneChip 430 array