Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

9 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ZEA

ABSTRACT: This is a cell culture based study to asses the impact of ZEA (Zearalenone) and E. coli co-contamination on IPEC cells, these is a normal epithelial cell line isolated from a new born piglet. ZEA is a mycotoxin with a negative impact in human health. The microarray is a custom Agilent Technology array slide with the AMAID: 05685.

ORGANISM(S): Sus scrofa

SUBMITTER: Cornelia Braicu

PROVIDER: E-MTAB-3885 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Zearalenone mycotoxin affects immune mediators, MAPK signalling molecules, nuclear receptors and genome-wide gene expression in pig spleen.

Pistol Gina Cecilia GC Braicu Cornelia C Motiu Monica M Gras Mihail Alexandru MA Marin Daniela Eliza DE Stancu Mariana M Calin Loredana L Israel-Roming Florentina F Berindan-Neagoe Ioana I Taranu Ionelia I

PloS one 20150526 5

The toxicity of zearalenone (ZEA) was evaluated in swine spleen, a key organ for the innate and adaptative immune response. Weaned pigs were fed for 18 days with a control or a ZEA contaminated diet. The effect of ZEA was assessed on wide genome expression, pro- (TNF-α, IL-8, IL-6, IL-1β, IFN-γ) and anti-inflammatory (IL-10, IL-4) cytokines, other molecules involved in inflammatory processes (MMPs/TIMPs), as well as signaling molecules, (p38/JNK1/JNK2-MAPKs) and nuclear receptors (PPARγ/NFkB/AP- ...[more]

PMID: 26011631

Similar Datasets

Expression profiling of the genes responding to zearalenone and its analogues using estrogen-responsive genes.

Project description:Comparing the expression profiles of the genes in response to estrogen or 17b-estradiol (E2) and a mycotoxin, zearalenone (ZEA), and its analogues. Keywords: Gene expression profiling Two-condition experiment, E2- or ZEA-treated vs. control cells. 2 to 3 biological replicates, independently grown and harvested. Two to three replicates per array.

2010-09-09 | E-GEOD-15249 | biostudies-arrayexpress

Expression data from LCM-captured Zea mays root cells after challenge with Meloidogyne incognita

Project description:The differentiation of specialized feeding sites in Zea mays root cells in response to nematode infestation involves substantial cellular reprogramming of host cells that is not well characterized at the molecular level. Expression data was generated from Zea mays root cells undergoing giant cell formation due to nematode infestation and from non-infested control root cells. Cells were laser captured 14 and 21 days after infestation. Each time point (14 day and 21 day) consisted of three biological replicates per treatment (control root cells or giant cells). Control cells were captured from an area ~13,000,000 um2 in size and giant cells were captured from an area ~5,000,000 um2 in size. RNA samples were isolated using the PicoPure RNA Isolation Kit (Arcturus, Mountain View, USA). RNA amplifications were carried out with the NuGEN WT-Ovation Pico kit.

2012-04-27 | E-GEOD-37556 | biostudies-arrayexpress

Dietary sterols/steroids and the generalist caterpillar Helicoverpa zea: physiology, biochemistry and midgut gene expression

Project description:Sterols are essential nutrients for insects because, in contrast to mammals, no insect (or arthropod for that matter) can synthesize sterols de novo. Cholesterol is the most common sterol in insects, but it is not found in plants in large quantities; plant-feeding insects typically generate their cholesterol by metabolizing phytosterols. However, different plants species can contain different types of phytosterols, and some phytosterols are not readily converted to cholesterol. In this study we examined, using artificial diets containing single sterols, how typical (cholesterol and stigmasterol) and atypical (cholestanol and cholestanone) sterols/steroids affect the performance of a generalist caterpillar (Helicoverpa zea), restricting this analysis to midgut tissue because this is where sterol/steroid absorption occurs, and the midgut is the putative site of dietary sterol/steroid metabolism. In general, H. zea performed best on the cholesterol and stigmasterol treatments; performance was reduced on cholestanol, and was very poor on cholestanone. We compared the transcript profiles of larval guts in response to differentially suitable sterols, using the optimal sterol, cholesterol, as a control, using a two-color reference design microarray experiment. Midgut gene expression patterns differed between the treatments; relative to cholesterol, differences were lowest on the stigmasterol treatment, intermediate on the cholestanol treatment, and greatest on the cholestanone treatment. Transcriptional profiling comparing Helicoverpa zea gut tissue from third instar larvae exposed to four different dietary sterols, namely Cholesterol (CON), Cholestanol (ChStanol), Cholestan-3-one (Ch3one) and Stigmasterol (Stigma). Two-color reference design. Biological replicates: 4 (5 individuals per replicate). 12 samples total.

2012-06-13 | E-GEOD-38699 | biostudies-arrayexpress

Transcriptome sequencing of and microarray development for a Helicoverpa zea cell line to investigate in vitro insect cell-baculovirus interactions

Project description:The Helicoverpa armigera single-capsid nucleopolyhedrovirus (HaSNPV) can be propagated using H. zea insect cell cultures, for use as a biopesticide against Heliothine agricultural pests.This study sequenced, assembled and functionally annotated 29,586 transcript sequences from cultured H. zea cells using Illumina 100 bps and paired-end transcriptome sequencing (RNA-seq). From these sequences, a genome-scale microarray platform was constructed and validated for effective expression analysis of H. zea genes. This array also included probes for all HaSNPV genes, thereby allowing virus and host gene changes to be monitored simultaneously. A 4x180,000 SurePrint Agilent expression array (Agilent, Santa Clara, CA) was employed so that a high number of probes can be included to test all 29,586 assembled H. zea sequences and to eventually select a best probe for each transcript. Two biological replicates for uninfected H. zea cells and two other biological replicates for infected samples at 18 hours post infection were analyzed. Six Agilent 60-mer oligonucleotide probes were designed by eArray (Agilent) for each transcript, in which each orientation had three probes randomly distributed across the sequences. Probes that had potential cross-hybridization (Xhyb) were removed. The final probe set for H. zea sequences included 153,583 probes. In addition, probes for all 135 H.armigera single-capsid nucleopolyhedrovirus (HaSNPV) genes were added to investigate host-virus interaction in culture.

2012-03-01 | E-GEOD-34418 | biostudies-arrayexpress

Assessment of zearalenone-induced cell survival and global gene regulation in mouse TM4 Sertoli cells

Project description:Zearalenone (ZEA) is a non-steroidal xenoestrogen mycotoxin produced by many Fusarium fungal species, which are common contaminants of cereal crops destined for worldwide human and animal consumption. ZEA has been linked to various male reproduction problems including decreased fertility potential. In this study, the direct impact of ZEA on the immature Sertoli TM4 cell line was evaluated. Results showed that high concentration of ZEA increase reactive oxygen species via the activation of MAPK signaling. Transcriptome analysis was performed on TM4 cell line treated with ZEA and genes involved in sex differentiation (Fgfr2, Igf1, Notch1, Sox9) and extracellular matrix (ECM) formation (Ctgf, Fam20a, Fbn1, Mmp9, Postn, Sparcl1, Spp1) were identified at the center of the functional protein association network suggesting that ZEA could be detrimental to the early steps of Sertoli cell differentiation.

2022-03-07 | GSE185111 | GEO

Early transcriptional changes in Zea mays seedlings exposed to Z-3-hexenol

Project description:Z-3-Hexenol and other green leaf volatiles have been known to induce defense-related gene expression. Here we investigated the early transcriptional changes in response to Z-3-hexenol. 3-week-old Zea mays seedling (inbred line B73) was exposed to pure Z-3-hexenol (1.5 Î¼M) in a glass cylinder. Controls were treated likewise without the addition of Z-3-hexenol. Plants were exposed for 20 min and 60 min. The second leaf was then cut and shock-frozen in liquid N2 and then stored at -85Â°prior to RNA extraction. 3 individual leaves were pooled for 1 biological replicate. 3 biological replicates were performed for each treatment, with one dye swap (second sample).

2013-06-15 | E-GEOD-47982 | biostudies-arrayexpress

Genome scale analysis of host-virus mRNA differential expression in Helicoverpa zea insect cells infected by H. armigera baculovirus from early till late infection

Project description:A custom microarray, based on deep transcriptome sequencing ((GEO accession number: GSE34418), was used to simultaneously investigate expression of over 24,000 Helicoverpa zea insect transcripts and 134 H. armigera nucleopolyhedrovirus (HearNPV) genes throughout the infection process at 0, 12, 24 and 48 hours post infection A cutom 8x60,000 SurePrint Agilent expression slide (Agilent, Santa Clara, CA) was used to analyzed eight samples, including biological replicates of HaSNPV-infected cultures at 0, 12, 24 and 48 hpi.The microarray probes included probes for 27,400 H. zea sequences that were validated previously (Nguyen et al., 2012. PLOS ONE, 7(5), e36324) and all 134 H. armigera single-capsid nucleopolyhedrovirus (HearNPV) genes (Accession: NC_002654).

2013-03-01 | E-GEOD-44184 | biostudies-arrayexpress

Transcriptional changes in Zea mays seedlings treated with insect elicitor (volicitin).

Project description:Insect elicitors, in particular fatty acid amides like volicitin, have been known to induce defense-related gene expression. Here we investigated transcriptional changes in response to volicitin 60min after treatment locally and in distal parts of the treated leaf. 3-Week old Zea mays seedling (inbred line B73) were treated with pure volicitin (1nmol per plant). Controls were untreated. Plants were for 60 min. The second leaf was then taken and a segment of 1 cm cut around the damage site and shock-frozen in liquid N2. 3 segments from 3 individual leaves were pooled for one biological replicate and then stored at -85M-BM-0prior to RNA extraction. For distal gene expression analysis a 1cm segment was cut about 3 cm leaf upwards from the volicitin application site and also frozen in liquid N2. 3 segments from 3 individual leaves were pooled for one biological replicate and then stored at -85M-BM-0prior to RNA extraction. 2 biological replicates were performed for each treatment with one dye swap (second sample).

2013-09-19 | E-GEOD-50981 | biostudies-arrayexpress

Expression profiling of the genes responding to zearalenone and its analogues using estrogen-responsive genes.

2010-03-11 | GSE15249 | GEO