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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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MicroRNA genetic variation, from population analysis to functional implications of allele variants associated with cancer

ABSTRACT: Total RNA samples from three independent transfection experiments of vectors containing two different allelic variants of 4 miRNAs (hsa-mir-146a, hsa-mir-196a2, hsa-mir-499a, hsa-mir-499b) and an empty vector. The selected variants are cancer associated SNPs located in cancer related miRNAs. The aim of this study is to identify functional explanations for those associations. HeLa cells were used for microarray expression experiments in Agilent SurePrint G3 Human Gene Expression microarrays (8x60k), which target over 60,000 well-annotated RefSeq transcripts starting from 300ng of total RNA. Data were analyzed using the Array File Maker (AFM) 4.0 software package (Array File Maker, Stanford, California).

ORGANISM(S): Homo sapiens

SUBMITTER: Ignasi Torruella

PROVIDER: E-MTAB-3990 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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