Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ChIP-chip by array of mouse ovaries from wild-type 5 week old Swiss mice using custom anit-Foxl2 polyclonal antibodies


ABSTRACT: ChIP-on-Chip experiment using chromatin from wild-type 5 weeks old swiss mice ovaries (Nishi et al, 2001) and an isovolumic blend of our custom anti-Foxl2 polyclonal antibodies (Cocquet J et al, 2002). Non precipitated sheared matched deproteinized chromatin (Input) was used a control to estimate genomic enrichment peaks (and thus Foxl2 binding sites) from IPed DNA. DNA from three independent ChIP assays (Input extractions) was pooled, and 100ng of DNA was linearly amplified using the Whole Genome Amplification kit (Sigma).

ORGANISM(S): Mus musculus

SUBMITTER: Berenice Benayoun 

PROVIDER: E-MTAB-400 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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