Molecular Profiling of Chemoresistant Slow Cycling Cancer-Initiating Cells from human colorectal cancer, glioma and melanoma.

ABSTRACT: SUMMARY: Dormant or slow cycling tumour cells can form a residual chemoresistant reservoir responsible for relapse in patients, years after curative surgery and adjuvant therapy. Since their isolation from human tumors has been a long-stalled technical challenge preventing a direct study of their functional properties, we have adapted the pulse-chase expression and labeling approach using H2BeGFP (Tumbar et al., 2004, DOI:10.1126/science.1092436) to a doxycycline-inducible all-in-one lentiviral vector. In this manner we were able to label and isolate by FACS live slow cycling cancer cells (SCCC) from human colorectal, glioma and melanoma patient-derived tumors as well as from minitumors grown in matrigel (SW1222-cells) or in sphere culture (e225, e216 glioma models). We determined the diff

INSTRUMENT(S): PC, MoFlo Legacy cell sorter (Beckman Coulter)

ORGANISM(S): Homo sapiens

SUBMITTER: Stephan Tenbaum 

PROVIDER: E-MTAB-4004 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress