Transcription profiling by array of mouse embryonic stem cell lines with Dgcr8 mutations to investigate the broad role of miRNAs and individual miRNAs through the reintroduction of miRNA mimics

ABSTRACT: These experiments were conducted as part of a study to derive the targets of miRNAs. Two independent BayGenomics mouse embryonic stem cell lines (XH157 and XG058), each bearing a gene trap between exons 9 and 10 of the Dgcr8 locus (ENSMUST00000115633, Ensembl v53) were further mutagenised by the targetted insertion of a second Dgcr8 gene trap cassette. Consequently two independent heterozygous and two independent homozygous mutant Dgcr8 cells were derived (Dgcr8tm1,gt1/+, Dgcr8tm1,gt2/+, Dgcr8gt1/tm1 and Dgcr8gt2/tm1). A comparison of the expression profiles of the heterozygous cells to the Dgcr8 depleted cells allows the investigation of the broad role of miRNAs in mouse ES cells. Subsequently individual miRNA mimics were reintroduced into the Dgcr8-depleted background. The cells were allowed to recover for 10, 20 or 44 hours. The effect of the miRNA on the mRNA expression of the cells was assessed through the comparison of the expression profile of the transfected cells to that of cells transfected with a control mimic, which had recovered over the same period. This allows the roles of individual miRNAs to be investigated.

ORGANISM(S): Mus musculus

SUBMITTER: Matthew Davis 

PROVIDER: E-MTAB-418 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress