Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Zea mays Epigenomics

ABSTRACT: RNA-directed DNA methylation (RdDM) in plants is a well-characterized example of RNA interference-related transcriptional gene silencing. To determine the relationships between RdDM and heterochromatin in the repeat-rich maize (Zea mays) genome, we performed whole-genome analyses of several heterochromatic features: dimethylation of lysine 9 and lysine 27 (H3K9me2 and H3K27me2), chromatin accessibility, DNA methylation, and small RNAs; we also analyzed two mutants that affect these processes, mediator of paramutation1 and zea methyltransferase2.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Zea mays

SUBMITTER: Kelly Dawe 

PROVIDER: E-MTAB-4191 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Accessible DNA and relative depletion of H3K9me2 at maize loci undergoing RNA-directed DNA methylation.

Gent Jonathan I JI   Madzima Thelma F TF   Bader Rechien R   Kent Matthew R MR   Zhang Xiaoyu X   Stam Maike M   McGinnis Karen M KM   Dawe R Kelly RK  

The Plant cell 20141202 12

RNA-directed DNA methylation (RdDM) in plants is a well-characterized example of RNA interference-related transcriptional gene silencing. To determine the relationships between RdDM and heterochromatin in the repeat-rich maize (Zea mays) genome, we performed whole-genome analyses of several heterochromatic features: dimethylation of lysine 9 and lysine 27 (H3K9me2 and H3K27me2), chromatin accessibility, DNA methylation, and small RNAs; we also analyzed two mutants that affect these processes, me  ...[more]

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