Differential RNA-seq for identifying toxin MazF and MqsR cleavage sites, beginning of transcripts and stationary phase cleavage sites in Escherichia coli (mRNA, rRNA and other ncRNA)

ABSTRACT: Bacterial toxin-antitoxin systems help bacteria to reduce their metabolism in various stressful conditions and also play a part in generating antibiotic tolerant bacterial subpopulation called persisters. Many toxins of the bacterial toxin-antitoxin systems are ribonucleases. Such toxins have been mostly viewed as degraders of mRNA, however recently it was demonstrated that they are also capable of cleaving non-coding RNA. Current libraries are a part of a project which aims to identify the major RNA cleavage sites (in mRNA, rRNA and regulatory non-coding RNA) of MazF and MqsR toxins in E. coli. We were also interested in the activity of promoters during toxin overexpression. We used a targeted RNA-sequencing approach that allowed us to map the distinct 5- and 3-ends of toxin-cleaved RNA a

INSTRUMENT(S): Infors HT Multitron shaker, Illumina HiSeq 2000

ORGANISM(S): Escherichia coli K-12

SUBMITTER: Niilo Kaldalu 

PROVIDER: E-MTAB-4204 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress