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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq analysis of Arabidopsis seedlings undergoing cold stress treatment

ABSTRACT: The goal of this project is to characterize splicing patterns and splice variants that occur under cold stress in plants.

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ketan Patel

PROVIDER: E-MTAB-4207 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A protocol for visual analysis of alternative splicing in RNA-Seq data using integrated genome browser.

Gulledge Alyssa A AA Vora Hiral H Patel Ketan K Loraine Ann E AE

Methods in molecular biology (Clifton, N.J.) 20140101

Ultrahigh-throughput sequencing of cDNA (RNA-Seq) is an invaluable resource for investigating alternative splicing in an organism. Alternative splicing is a form of posttranscriptional regulation in which primary RNA transcripts from a single gene can be spliced in multiple ways leading to different RNA and protein products. In plants and other species, it has been shown that many genes involved in circadian regulation are alternatively spliced. As new RNA-Seq data sets become available, these d ...[more]

PMID: 24792048

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