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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Oryza sativa Transcriptome or Gene expression

ABSTRACT: To investigate impacts of genome-wide loss of Cytosine methylation at CG sites (mCs) on global expression of protein-coding genes (henceforth referring to genes), we conducted deep RNA-seq analysis on OsMET1-2â/â relative to its sibling WT and heterozygote (OsMET1-2+/â), each with two biological replicates

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Oryza sativa

SUBMITTER: Bao Liu

PROVIDER: E-MTAB-4224 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Mutation of a major CG methylase in rice causes genome-wide hypomethylation, dysregulated genome expression, and seedling lethality.

Hu Lanjuan L Li Ning N Xu Chunming C Zhong Silin S Lin Xiuyun X Yang Jingjing J Zhou Tianqi T Yuliang Anzhi A Wu Ying Y Chen Yun-Ru YR Cao Xiaofeng X Zemach Assaf A Rustgi Sachin S von Wettstein Diter D Liu Bao B

Proceedings of the National Academy of Sciences of the United States of America 20140707 29

Cytosine methylation at CG sites ((m)CG) plays critical roles in development, epigenetic inheritance, and genome stability in mammals and plants. In the dicot model plant Arabidopsis thaliana, methyltransferase 1 (MET1), a principal CG methylase, functions to maintain (m)CG during DNA replication, with its null mutation resulting in global hypomethylation and pleiotropic developmental defects. Null mutation of a critical CG methylase has not been characterized at a whole-genome level in other hi ...[more]

PMID: 25002488

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