Transcription profiling by array of the secretome from human myotube and myotube derived vesicles

ABSTRACT: Human satellite cells were isolated from muscle biopsies and expanded in vitro. Cells were induced to differentiate into branched multinucleated myotubes by serum depletion and were harvested from three independent cultures after 72 hours. Total RNA was isolated, amplified, labelled and hybridised to Illumina Human WG-6v3 arrays. Data were analysed to identify a "virtual secretome". These data and proteomic data from similar cultures suggested that differentiating muscle cultures produce microvesicles - which were characterised in the ensuing experiment. Human satellite cells were cultured as before (duplicate cultures) and culture supernatants were subjected to a differential centrifugation procedure, resulting in isolation of two distinct vesicle populations: exosomes and micropart

ORGANISM(S): Homo sapiens

SUBMITTER: Marie-Catherine Le Bihan 

PROVIDER: E-MTAB-423 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress