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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of the secretome from human myotube and myotube derived vesicles

ABSTRACT: Human satellite cells were isolated from muscle biopsies and expanded in vitro. Cells were induced to differentiate into branched multinucleated myotubes by serum depletion and were harvested from three independent cultures after 72 hours. Total RNA was isolated, amplified, labelled and hybridised to Illumina Human WG-6v3 arrays. Data were analysed to identify a "virtual secretome". These data and proteomic data from similar cultures suggested that differentiating muscle cultures produce microvesicles - which were characterised in the ensuing experiment. Human satellite cells were cultured as before (duplicate cultures) and culture supernatants were subjected to a differential centrifugation procedure, resulting in isolation of two distinct vesicle populations: exosomes and microparticles. Total RNA was isolated from these vesicle populations and the cells from which they were derived. RNA was amplified, labelled and hybridised to Illumina Human HT12v3 arrays. Data from myotubes, exosomes and microparticles were analysed to identify transcripts present, differential expression and functional interpretation of transcriptomes. IDAT files are available on the ArrayExpress ftp site in the additional.zip file.

ORGANISM(S): Homo sapiens

SUBMITTER: Marie-Catherine Le Bihan

PROVIDER: E-MTAB-423 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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