Gene Mapping via Bulked Segregant RNA-Seq (BSR-Seq)

ABSTRACT: Bulked segregant analysis (BSA) is an efficient method to rapidly and efficiently map genes responsible for mutant phenotypes. This procedure, however, requires access to quantitative genetic markers that are polymorphic in the mapping population. We have developed a modification of BSA (BSR-Seq) that makes use of RNA-Seq reads to efficiently map genes even in populations for which no polymorphic markers have been previously identified. Because of the digital nature of next-generation sequencing (NGS) data, it is possible to conduct de novo SNP discovery and quantitatively genotype BSA samples using the same RNA-Seq data. In addition, analysis of the RNA-Seq data provides information on the effects of the mutant on global patterns of gene expression at no extra cost. In combination these r

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Zea mays

SUBMITTER: Schnable Patrick 

PROVIDER: E-MTAB-4234 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress