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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq analysis of Arabidopsis thaliana esr1-1 mutant versus wild-type (JC66)

ABSTRACT: A GSTF8 promoter fragment fused to the luciferase reporter gene was used in a forward genetic screen for Arabidopsis mutants with up-regulated GSTF8 promoter activity and identified the esr1-1 (enhanced stress response 1) mutant which also conferred increased resistance to the fungal pathogen Fusarium oxysporum. RNA-Seq was conducted to compare esr1-1 and wild-type (GSTF8 promoter::luciferase reporter line JC66 in Col-0 background) transcriptomes for altered gene expression.

INSTRUMENT(S): Illumina HiSeq 1000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Louise Thatcher

PROVIDER: E-MTAB-4235 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The Arabidopsis KH-Domain RNA-Binding Protein ESR1 Functions in Components of Jasmonate Signalling, Unlinking Growth Restraint and Resistance to Stress.

Thatcher Louise F LF Kamphuis Lars G LG Hane James K JK Oñate-Sánchez Luis L Singh Karam B KB

PloS one 20150518 5

Glutathione S-transferases (GSTs) play important roles in the protection of cells against toxins and oxidative damage where one Arabidopsis member, GSTF8, has become a commonly used marker gene for early stress and defense responses. A GSTF8 promoter fragment fused to the luciferase reporter gene was used in a forward genetic screen for Arabidopsis mutants with up-regulated GSTF8 promoter activity. This identified the esr1-1 (enhanced stress response 1) mutant which also conferred increased resi ...[more]

PMID: 25985302

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Transcription profiling by high throughput sequencing of Arabidopsis thaliana esr1-1 mutant versus wild-type (JC66)

Project description:A GSTF8 promoter fragment fused to the luciferase reporter gene was used in a forward genetic screen for Arabidopsis mutants with up-regulated GSTF8 promoter activity and identified the esr1-1 (enhanced stress response 1) mutant which also conferred increased resistance to the fungal pathogen Fusarium oxysporum. RNA-Seq was conducted to compare esr1-1 and wild-type (GSTF8 promoter::luciferase reporter line JC66 in Col-0 background) transcriptomes for altered gene expression.

2016-04-28 | E-MTAB-4235 | ExpressionAtlas

Role of fungal cellulases upon plant infection

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esr1-drn-Transcriptomic study of the transcription factor ESR1/DRN.

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esr1-drn-Transcriptomic study of the transcription factor ESR1/DRN.

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ChIP-Seq on ESR1 in human liver tissue and hepatocytes

Project description:To improve our understanding of transcriptional regulation by ESR1 in the liver, chromatin immunoprecipitation with an antibody against ESR1 followed by high-throughput sequencing (ESR1 ChIP-Seq) was conducted in human liver samples and in hepatocytes with or without 17beta-estradiol (E2) treatment. By comparing treated and untreated hepatocytes, we identified both ligand-dependent and ligand-independent binding sites throughout the genome. Ligand-dependent binding sites include ChIP-Seq peaks that either appeared (gained peaks) or disappeared (lost peaks) upon estrogen treatment. Gained peaks occurred at the Estrogen Response Element (ERE) whereas the sites for lost peaks were instead coenriched with a variety of transcription factors. In both cases, ESR1 binding primarily occurred in enhancer regions and was associated with general liver functions, such as lipid/energy metabolism. In contrast, we also observed a subset of ESR1 sites that were maintained regardless of estrogen treatment. These ligand-independent sites mostly occurred at promoter regions and were highly enriched with several cofactor motifs.

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Comparative O-glycoproteomics of Fusarium oxysporum

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Genome Sequencing and Comparative Transcriptomics of Race1 and Race4 of Banana pathogen: Fusarium oxysporum f.sp. Cubense

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New insights into antifungal resistance mechanisms in Fusarium species revealed by high throughput sequencing

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Transcriptome analysis reveals the response mechanism of Frl-mediated resistance to Fusarium oxysporum f. sp. radicis-lycopersici (FORL) infection in tomato

Project description:Transcriptome analysis reveals the response mechanism of Frl-mediated resistance to Fusarium oxysporum f. sp. radicis-lycopersici (FORL) infection in tomato

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