Genome annotation improvement of Brassica rapa

ABSTRACT: The mapping and functional analysis of quantitative traits in Brassica rapa can be greatly improved with the availability of physically positioned, gene-based genetic markers and accurate genome annotation. In this study, deep transcriptome RNA sequencing (RNA-Seq) of Brassica rapa was undertaken with two objectives: SNP detection and improved transcriptome annotation. We performed SNP detection on two varieties that are parents of a mapping population to aid in development of a marker system for this population and subsequent development of high-resolution genetic map. An improved Brassica rapa transcriptome was constructed to detect novel transcripts and to improve the current genome annotation. Deep RNA-Seq of two Brassica rapa genotypes—R500 (var. trilocularis, Yellow Sarson) and IMB

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Brassica rapa

SUBMITTER: Julin Maloof 

PROVIDER: E-MTAB-4276 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress