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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Epigenetic memory mediated by HDA6, Pol IV and Pol V in Arabidopsis.

ABSTRACT: Multiple RNA-sequencing datasets were generated from wild-type Col-0 and hda6, pol IV or pol V null mutant samples to dissect genetic requirements for the maintenance of epigenetic memory and silent locus identity in Arabidopsis thaliana.

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Craig Pikaard

PROVIDER: E-MTAB-4337 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A two-step process for epigenetic inheritance in Arabidopsis.

Blevins Todd T Pontvianne Frédéric F Cocklin Ross R Podicheti Ram R Chandrasekhara Chinmayi C Yerneni Satwica S Braun Chris C Lee Brandon B Rusch Doug D Mockaitis Keithanne K Tang Haixu H Pikaard Craig S CS

Molecular cell 20140320 1

In Arabidopsis, multisubunit RNA polymerases IV and V orchestrate RNA-directed DNA methylation (RdDM) and transcriptional silencing, but what identifies the loci to be silenced is unclear. We show that heritable silent locus identity at a specific subset of RdDM targets requires HISTONE DEACETYLASE 6 (HDA6) acting upstream of Pol IV recruitment and siRNA biogenesis. At these loci, epigenetic memory conferring silent locus identity is erased in hda6 mutants such that restoration of HDA6 activity ...[more]

PMID: 24657166

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