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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Selaginella moellenforfii, Arabidopsis thaliana, Equisetum arvense, Zea mays shoot meristems transcriptome

ABSTRACT: We used LM-RNAseq to compare the molecular fingerprints of cells enriched for subdomains within Selaginella, Equisetum, Arabidopsis and maize shoot apices. Three apical domains were isolated from the Selaginella and Equisetum SAMs: the AC domain, comprising the lone AC; the core domain, comprising the cells below the AC and above the initiating leaf primordium; and the initiating leaf primordium. LM-RNAseq analyses of these shoot apical subdomains generated hundreds of significantly DEGs for each cell type relative to whole-plant transcriptomes based on an FDR â¤ 0.05. These data were analyzed for the presence of homologous developmental genetic programs across these three species, and for the identification of unique developmental programs operating within each species.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Selaginella moellendorffii

SUBMITTER: Michael Scalon

PROVIDER: E-MTAB-4374 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dissecting the molecular signatures of apical cell-type shoot meristems from two ancient land plant lineages.

Frank Margaret H MH Edwards Molly B MB Schultz Eric R ER McKain Michael R MR Fei Zhangjun Z Sørensen Iben I Rose Jocelyn K C JK Scanlon Michael J MJ

The New phytologist 20150421 3

Shoot apical meristem (SAM) structure varies markedly within the land plants. The SAMs of many seedless vascular plants contain a conspicuous inverted, pyramidal cell called the apical cell (AC), which is unidentified in angiosperms. In this study, we use transcriptomic sequencing with precise laser microdissections of meristem subdomains to define the molecular signatures of anatomically distinct zones from the AC-type SAMs of a lycophyte (Selaginella moellendorffii) and a monilophyte (Equisetu ...[more]

PMID: 25900772

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