Unknown,Transcriptomics,Genomics,Proteomics

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Mapping Gene Activity of Arabidopsis Root Hairs


ABSTRACT: We provide an atlas of gene and protein expression in Arabidopsis root hair cells, generated by paired-end RNA-seq and LC/MS-MS analysis from protoplasts that carry the root hair-specific pEXP7-GFP reporter construct. In total, transcripts from 23,234 genes were detected in root hairs; those related to cell wall biosynthesis and translation differed most dramatically in abundance when compared to non-GFP root protoplasts.

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ping Lan 

PROVIDER: E-MTAB-4375 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Mapping gene activity of Arabidopsis root hairs.

Lan Ping P   Li Wenfeng W   Lin Wen-Dar WD   Santi Simonetta S   Schmidt Wolfgang W  

Genome biology 20130625 6


<h4>Background</h4>Quantitative information on gene activity at single cell-type resolution is essential for the understanding of how cells work and interact. Root hairs, or trichoblasts, tubular-shaped outgrowths of specialized cells in the epidermis, represent an ideal model for cell fate acquisition and differentiation in plants.<h4>Results</h4>Here, we provide an atlas of gene and protein expression in Arabidopsis root hair cells, generated by paired-end RNA sequencing and LC/MS-MS analysis  ...[more]

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