Transcriptome-based association genetics in hexaploid wheat

ABSTRACT: Many crop species have complex genomes, making the conventional pathway to associating molecular markers with trait variation, which includes genome sequencing, both expensive and time-consuming. We used a streamlined approach to rapidly develop a genomics platform for hexaploid wheat based on the inferred order of expressed sequences. This involved assembly of the transcriptomes for the progenitor genomes of bread wheat, the development of a genetic linkage map comprising 9495 mapped transcriptome-based SNP markers, use of this map to rearrange the genome sequence of Brachypodium distachyon into pseudomolecules representative of the genome organization of wheat and sequence similarity-based mapping onto this resource of the transcriptome assemblies. To demonstrate that this approximatio

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Triticum dicoccoides

SUBMITTER: Robert Davey 

PROVIDER: E-MTAB-4414 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress