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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomic characterization of cattle primary hepatocytes and its comparison with Madin-Darby bovine kidney (MDBK) cells and liver tissue

ABSTRACT: Cattle primary cultured hepatocytes (CHs) have been used as a useful in vitro model for drug metabolism studies. However, they suffer some limitations, when compared with the ex vivo liver tissue, such as difficult adhesion to the substrate following isolation, a limited viability and a rapid loss of differentiated characteristics. Therefore, a reliable hepatic in vitro model is actually needed for cattle. As established bovine hepatic cell lines are not available, we characterized the cattle primary CHs' transcriptome by using a cDNA microarray approach and compared it with ex vivo cattle liver tissue as well as with the Madin-Darby Bovine Kidney (MDBK) cells (as an established cell line for transfection purposes).
This part of the study compares the primary CHs vs. liver tissue. The comparison of MDBK cells vs. the primary CHs is found under E-MTAB-4430.

INSTRUMENT(S): Agilent G2565BA scanner, Agilent Hybridization Oven

ORGANISM(S): Bos taurus

SUBMITTER: Ramy Elgendy

PROVIDER: E-MTAB-4424 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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