Molecular anatomy of gene expression responses in stressed hepatocytes

ABSTRACT: It is well-known that isolation and cultivation of primary hepatocytes causes major gene expression alterations. In the present genome-wide, time resolved study of cultivated human and mouse hepatocytes, we made the observation that expression changes in culture strongly resemble alterations in liver diseases. Hepatocytes of both species were cultivated in collagen sandwich and in monolayer conditions. Genome-wide data were also obtained from human NAFLD, cirrhosis, HCC, and hepatitis B virus infected tissue as well as mouse livers after partial hepatectomy, CCl4 intoxication, obesity, HCC and LPS. A strong similarity between cultivation and disease induced expression alterations was observed. For example, expression changes in hepatocytes induced by one day cultivation and one day CCl4 ex

ORGANISM(S): Mus musculus

SUBMITTER: Jan Hengstler 

PROVIDER: E-MTAB-4444 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress