MRNA time-course transcription profiling of bacteriocytes and gut tissue of pea aphids reared on standard artificial diet (AP3) and artificial diet depleted in Tyr and Phe (YFØ)

ABSTRACT: Tyrosine (Tyr, Y) and phenylalanine (Phe, F) synthesis is shared by the pea aphid and its symbiont Buchnera aphidicola.These aromatic amino acids are essential for the pea aphid growth and development. To characterize the molecular mechanisms, at gene transcriptional level, underlying this symbiotic integrated network pea aphids (Acyrthosyphon pisum, clone LL01) were reared on (i) standard artificial diet (AP3) and (ii) on the same AP3 medium depleted of Tyr (Y) and Phe (F). From each of the two groups, aphids were collected at specific time points and dissected: 12 h (D0), 1 day (D1), 2 days (D2), 3 days (D3), 4 days (D4), 5 days (D5) and 7 days (D7). Total RNA, to be used in gene expression analysis by arrays, was extracted, under the two rearing conditions, from two tissues: gut [from

ORGANISM(S): Acyrthosiphon pisum

SUBMITTER: Stefano Colella 

PROVIDER: E-MTAB-4456 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress