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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional profiling of human naive embryonic stem cells

ABSTRACT: Human pluripotent cell lines were derived from blastocyst-stage embryos and propagated in self-renewal conditions that maintain features of naive pluripotency characteristic of mouse embryonic stem cells. Transcriptional activity of HNES1, HNES2 and HNES3 cell lines was assessed with RNA-seq.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Paul Bertone

PROVIDER: E-MTAB-4461 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Naive Pluripotent Stem Cells Derived Directly from Isolated Cells of the Human Inner Cell Mass.

Guo Ge G von Meyenn Ferdinand F Santos Fatima F Chen Yaoyao Y Reik Wolf W Bertone Paul P Smith Austin A Nichols Jennifer J

Stem cell reports 20160303 4

Conventional generation of stem cells from human blastocysts produces a developmentally advanced, or primed, stage of pluripotency. In vitro resetting to a more naive phenotype has been reported. However, whether the reset culture conditions of selective kinase inhibition can enable capture of naive epiblast cells directly from the embryo has not been determined. Here, we show that in these specific conditions individual inner cell mass cells grow into colonies that may then be expanded over mul ...[more]

PMID: 26947977

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Karyotype analysis of human naive embryonic stem cells

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2016-03-01 | E-MTAB-4463 | biostudies-arrayexpress

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Domains of genomewide gene expression dysregulation in Down syndrome

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Single cell Hi-C reveals cell-to-cell variability in chromosome structure

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2013-09-26 | E-GEOD-48262 | biostudies-arrayexpress

PAR-CLIP-seq reveals RNAs directly interacting with CTCF in human transformed cell line U2OS

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2014-03-18 | E-GEOD-53554 | biostudies-arrayexpress

PAR-CLIP for JARID2 in mouse embryonic stem cells

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