Experimental identification of small non-coding RNAs in the model marine bacterium Ruegeria pomeroyi DSS-3

ABSTRACT: In oligotrophic ocean waters where bacteria are often subjected to chronic nutrient limitation, community transcriptome sequencing has pointed to the presence of highly abundant small RNAs (sRNAs). The role of sRNAs in regulating response to nutrient stress was investigated in a model heterotrophic marine bacterium Ruegeria pomeroyi grown in continuous culture under carbon and nitrogen limitation. RNAseq analysis identified 98 sRNAs, of which 69 were cis-encoded and located antisense to their target genes, and 30 were trans-encoded and linked to predicted target genes through complementarity analysis. The most prevalent functional roles of target genes were transport, cell-cell interactions, signal transduction, and transcriptional regulation. Thirty-two percent of the sRNAs had been ident

INSTRUMENT(S): AB SOLiD 4 System

ORGANISM(S): Ruegeria pomeroyi DSS-3

SUBMITTER: Adam Rivers 

PROVIDER: E-MTAB-4468 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress