Transcript residency on ribosomes reveals a key role for the Arabidopsis thaliana bundle sheath in sulphur and glucosinolate metabolism.

ABSTRACT: Leaves of angiosperms are made up of multiple distinct cell types. While the function of mesophyll cells, guard cells, phloem companion cells and sieve elements are clearly described, this is not the case for the bundle sheath (BS). To provide insight into the role of the BS in the C3 species Arabidopsis thaliana, we labelled ribosomes in this cell type with a FLAG tag. We then used immunocapture to isolate these ribosomes followed by sequencing of resident mRNAs. This showed that 5% of genes showed specific splice forms in the BS, and that 15% of genes were preferentially expressed in these cells. The BS translatome strongly implicates the BS in specific roles in sulphur transport and metabolism, glucosinolate biosynthesis and trehalose metabolism. Much of the C4 cycle is differential

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Richard Smith-Unna 

PROVIDER: E-MTAB-4474 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress