Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

RNA-Seq of Bacillus cereus strains ATCC 10987, ATCC 14579 at multiple time-points


ABSTRACT: Global mRNA decay in B. cereus

ORGANISM(S): Bacillus cereus

SUBMITTER: Ole Okstad 

PROVIDER: E-MTAB-450 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Global mRNA decay analysis at single nucleotide resolution reveals segmental and positional degradation patterns in a Gram-positive bacterium.

Kristoffersen Simen M SM   Haase Chad C   Weil M Ryan MR   Passalacqua Karla D KD   Niazi Faheem F   Hutchison Stephen K SK   Desany Brian B   Kolstø Anne-Brit AB   Tourasse Nicolas J NJ   Read Timothy D TD   Økstad Ole Andreas OA  

Genome biology 20120426 4


<h4>Background</h4>Recent years have shown a marked increase in the use of next-generation sequencing technologies for quantification of gene expression (RNA sequencing, RNA-Seq). The expression level of a gene is a function of both its rate of transcription and RNA decay, and the influence of mRNA decay rates on gene expression in genome-wide studies of Gram-positive bacteria is under-investigated.<h4>Results</h4>In this work, we employed RNA-Seq in a genome-wide determination of mRNA half-live  ...[more]

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