Monitoring global differential gene expression in context of protein thiol-oxidation and protein S-mycothiolation in Mycobacterium smegmatis str. MC2 155 under hypochlorite stress.

ABSTRACT: Mycobacterium smegmatis mc2 155 wild type strain was grown in triplicate in modified Hartmans-de Bont minimal medium (HdB) at 37C and vigorous agitation. Cultures were exposed to 1 mM NaOCl at an optical density at 500 nm (OD500) of 0.4 0.45 in the early log phase. M. smegmatis mc2 155 cells of 3 replicate experiments were harvested before and 30 min after exposure to 1 mM NaOCl and disrupted in 3 mM EDTA/ 200 mM NaCl lysis buffer with a Precellys24 Ribolyzer. RNA isolation was performed using phenol-chloroform-isoamylalcohol approach. After precipitation with 3 M sodium acetate and isopropanol, the total RNA was washed with cold ethanol and the pellet was solved in sterile water. Initially RNA quality was checked by Trinean Xpose (Gentbrugge,Belgium) and Agilent RNA Nano 6000 kit on Ag

INSTRUMENT(S): Illumina HiSeq 1500, Illumina MiSeq

ORGANISM(S): Mycobacterium smegmatis str. MC2 155

SUBMITTER: Tobias Busche 

PROVIDER: E-MTAB-4522 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress