Slicer activity of Arabidopsis AGO1 in siRNA biogenesis and miRNA target decay

ABSTRACT: Aim of study The objective of these studies was to analyze the implication of slicer activity of AGO1 in the following molecular processes: loading of siRNAs and miRNAs stability of siRNAs and miRNAs formation of RDR6/SGS3-dependent secondary siRNAs decay of mRNAs targeted by miRNAs The studies use point mutants defective in slicer activity and analyses total and AGO1-bound small RNA populations as well as mRNA populations in these mutants by next-generation sequencing.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Laura Arribas-Hernandez 

PROVIDER: E-MTAB-4529 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress