Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

The Global Molecular Landscape of dormant Hematopoietic Stem Cells

ABSTRACT: Rare dormant hematopoietic stem cells (dHSCs) reside at the top of the blood hierarchy harboring the highest long-term reconstitution capacity. However, no markers exist to prospectively identify dHSCs and their molecular identity, as well as the mechanism leading to their activation remains poorly understood. Here, we present the global transcriptome of ex vivo isolated mouse multipotent hematopoietic stem cells (HSCs) and dHSCs at the single cell level.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Florian Buettner 

PROVIDER: E-MTAB-4547 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Dormant hematopoietic stem cells (dHSCs) are atop the hematopoietic hierarchy. The molecular identity of dHSCs and the mechanisms regulating their maintenance or exit from dormancy remain uncertain. Here, we use single-cell RNA sequencing (RNA-seq) analysis to show that the transition from dormancy toward cell-cycle entry is a continuous developmental path associated with upregulation of biosynthetic processes rather than a stepwise progression. In addition, low Myc levels and high expression of  ...[more]

Similar Datasets

Transcriptomics RNA Seq of hematopoietic stem and progenitor cells
2017-05-18 | E-MTAB-4549 | biostudies-arrayexpress
Transcriptomics The Global Molecular Landscape of Hematopoietic Stem Cells and their Immediate Progeny
2014-08-21 | E-MTAB-2262 | biostudies-arrayexpress
Transcriptomics Comparison of aged dormant and active hematopoietic stem cells
2020-06-25 | E-MTAB-7903 | biostudies-arrayexpress
Transcriptomics RNA seq of CD45.2+ Neo1 deficient or wildtype Hematopoietic stem cells, 4 or 15 month after stem cell transplantation
2020-07-22 | E-MTAB-7902 | biostudies-arrayexpress
Transcriptomics Downstream Targets of HOXB4 in the primitive hematopoietic progenitor EML Cell line
2010-04-20 | E-GEOD-20604 | biostudies-arrayexpress
Transcriptomics ATAC-seq of LT-HSCs and MPPs
2021-11-02 | E-MTAB-9779 | biostudies-arrayexpress
Transcriptomics Gene expression comparisons of megakaryocyte-erythrocyte progenitors isolated from wild type and c-MybTG/c-MybTG mice
2011-01-10 | E-GEOD-19901 | biostudies-arrayexpress
Transcriptomics ATAC-seq of control and Cyp26b1KO HSCs
2021-11-02 | E-MTAB-9778 | biostudies-arrayexpress
Transcriptomics ATAC-seq of HSCs in Vitamin A-free diet
2021-11-02 | E-MTAB-9780 | biostudies-arrayexpress
Transcriptomics RNA Seq of wildtype and Rarb KO HSCs after 24h of in vitro culture and treatment
2021-11-02 | E-MTAB-9749 | biostudies-arrayexpress