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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Testis-to-ovary reprogramming and testis regression after ablation of Sox9 in Sox8-/- mice

ABSTRACT: We used two Sertoli-cell-specific Cre lines: Wt1-CreERT2 and Sox9-CreERT2, to induce Sox9 ablation on a Sox8 -/- background in the adult testis. Sox9/8 double KO testes undergo testis-to-ovary genetic reprogramming and Sertoli-to-granulosa cell transdifferentiation.

INSTRUMENT(S): HPC services from the University of Granada (http://alhambra.ugr.es), Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Miguel Burgos

PROVIDER: E-MTAB-4572 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sox9 and Sox8 protect the adult testis from male-to-female genetic reprogramming and complete degeneration.

Barrionuevo Francisco J FJ Hurtado Alicia A Kim Gwang-Jin GJ Real Francisca M FM Bakkali Mohammed M Kopp Janel L JL Sander Maike M Scherer Gerd G Burgos Miguel M Jiménez Rafael R

eLife 20160621

The new concept of mammalian sex maintenance establishes that particular key genes must remain active in the differentiated gonads to avoid genetic sex reprogramming, as described in adult ovaries after Foxl2 ablation. Dmrt1 plays a similar role in postnatal testes, but the mechanism of adult testis maintenance remains mostly unknown. Sox9 and Sox8 are required for postnatal male fertility, but their role in the adult testis has not been investigated. Here we show that after ablation of Sox9 in ...[more]

PMID: 27328324

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