Project description:Separate transcription profiling of oocytes and granulosa cells for each follicle stage: primordial (PD), primary (PM), secondary (SC) follicles and the small antral stage (SA) obtained by Laser Capture Microdissection (LCM) and RNAseq. The purpose of this study was to describe global gene expression during early ovarian folliculogenesis for each follicular compartment, to identify differential and specific gene expression between the 2 follicular compartments and during follicular development, to investigate specific function and pathways and to explore bi-directional communication between oocytes and GC.
Project description:Single cell RNA sequencing (10x) analysis of bovine in vitro meat with self-assembled muscle and neuronal tissue structures derived from bovine embryonic stem cells. Comparison of bovine in vitro meat from 2-D or 3-D culture methods. Bovine in vitro meat with and without co-induction of endothelial cells was also compared.
Project description:Transcriptional profiling of Bovine skeletal muscle was conducted comparing age of cattle and dietary regimes 4 prenatal time points, 4 postnatal time points, 2 dietary regimes at 3 time points
Project description:Obesity is a metabolic disease caused by environmental, genetic, and epigenetic factors. However, the epigenetic mechanisms of obesity are incompletely understood. The aim of our study was to identify skeletal muscle DNA methylation patterns in obesity. Muscle biopsies were obtained basally from lean (n=11) and obese (n=9) participants in combination with euglycemic hyperinsulinemic clamps to assess insulin sensitivity. We performed reduced representation bisulfite sequencing next generation methylation analysis on DNA isolated from vastus lateralis muscle biopsies.
Project description:The palmitoyl-proteome of bovine granulosa cells (GC) cells was investigated by combining acyl-biotin exchange chemistry and quantitative mass spectrometry analysis.
Project description:The palmitoyl-proteome of bovine cumulus-oocyte complexes (COCs) cells was investigated by combining acyl-biotin exchange chemistry and quantitative mass spectrometry analysis.