Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Bernardo mesoderm differentiation

ABSTRACT: Transcriptional profiling of hESCs in chemically-defined culture media compared to hESCs differentiated for 36h in the additional presence of FGF, LY294002 and either BMP or ActivinA.

ORGANISM(S): Homo sapiens

SUBMITTER: Matthew Trotter 

PROVIDER: E-MTAB-464 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages.

Bernardo Andreia S AS   Faial Tiago T   Gardner Lucy L   Niakan Kathy K KK   Ortmann Daniel D   Senner Claire E CE   Callery Elizabeth M EM   Trotter Matthew W MW   Hemberger Myriam M   Smith James C JC   Bardwell Lee L   Moffett Ashley A   Pedersen Roger A RA  

Cell stem cell 20110801 2

BMP is thought to induce hESC differentiation toward multiple lineages including mesoderm and trophoblast. The BMP-induced trophoblast phenotype is a long-standing paradox in stem cell biology. Here we readdressed BMP function in hESCs and mouse epiblast-derived cells. We found that BMP4 cooperates with FGF2 (via ERK) to induce mesoderm and to inhibit endoderm differentiation. These conditions induced cells with high levels of BRACHYURY (BRA) that coexpressed CDX2. BRA was necessary for and prec  ...[more]

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