Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

15 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of down stream genes in dehydration treated NCED3 mutants

ABSTRACT: To understand the Abscisic Acid (ABA) signaling in response to dehydration stress, we performed analysis of gene expression using Arabidopsis wild-type plants and the nced3-2 mutant under dehydration stress. The nced3-2 mutant is an Arabidopsis T-DNA tagged knock-out mutant of the NCED3 gene, which has an essential role in dehydration-inducible ABA biosynthesis. Arabidopsis plants were grown in in soil (verdenite 40 mmÎ¦, Verde Co., Ltd., Kanagawa, Japan) in a cell strainer (Falcon, 40 Î¼m; Corning Inc., NY, USA). Plants were grown at 22Â°C for 3 weeks under illumination (40â60 Î¼mol m-2s-1; 16 h light/8 h darkness). Three-week-old plants were exposed to dehydration stress by being denied water for 6, 24, 48, or 72 h.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Kyonoshin Maruyama

PROVIDER: E-MTAB-4640 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Identification of heat stress-responsive genes in soybean

Project description:To characterize heat stress-responsive genes and to clarify the heat stress-responsive transcription pathways, transcriptome analysis of soybean was conducted using microarray. Soybean (Glycine max cv. Williams82) were grown in plastic pots filled with nutrient soil for 2 weeks with a 12 h light (28Â°C)/12 h dark (25Â°C) regimen (ca. 1500 Î¼mol photons mâ2 sâ1) and were treated for 30 min at 42Â°C.

2017-02-23 | E-MTAB-4504 | biostudies-arrayexpress

Identification of HS-responsive genes in rice

Project description:To characterize plant heat stress-responsive genes and to clarify the heat stress-responsive transcription pathways, the transcriptome analysis of rice was conducted using microarray. Rice (Oryza sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks with a 12 h light (28Â°C)/12 h dark (25Â°C) regimen (ca. 1500 Î¼mol photons mâ2 sâ1) and were treated for 30 min at 42Â°C.

2017-02-23 | E-MTAB-4503 | biostudies-arrayexpress

Identification of down stream genes in heat treated NCED3 mutants

Project description:To characterize plant HS-responsive genes, the transcriptome analysis of rice was conducted using microarray. Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07â1.08) under a 16 h light/8 h dark photoperiod (50 Â± 10 Î¼mol photons mâ2 sâ1) at 22Â°C, and were treated for 30 min at 37Â°C.

2017-02-23 | E-MTAB-4506 | biostudies-arrayexpress

Transcription profiling by array of heat-stressed Arabidopsis plants

Project description:To characterize plant heat stress-responsive genes and to clarify the heat stress-responsive transcription pathways, the transcriptome analysis of rice was conducted using microarray. Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07â1.08) under a 16 h light/8 h dark photoperiod (50 Â± 10 Î¼mol photons mâ2 sâ1) at 22Â°C, and were treated for 30 min at 37Â°C.

2017-02-28 | E-MTAB-4531 | biostudies-arrayexpress

Transcription profiling by array of heat-stressed maize plants

Project description:To characterize plant heat stress-responsive genes and to clarify the heat stress-responsive transcription pathways, the transcriptome analysis of maize was conducted using microarray. Maize (Zea mays cv. B73) were grown in plastic pots filled with nutrient soil for 2 weeks with a 12 h light (28Â°C)/12 h dark (25Â°C) regimen (ca. 1500 Î¼mol photons mâ2 sâ1) and were treated for 30 min at 42Â°C.

2017-02-28 | E-MTAB-4530 | biostudies-arrayexpress

Transcription profiling by array of control and TCP13-SRDX transgenic Arabidopsis plants

Project description:To understand the downstream genes of TCP13, we performed analysis of gene expression using vector control plants and the TCP13 fused repression domain plants (TCP13pro::TCP13SRDX) . TheTCP13pro::TCP13SRDX plants is transgenic plants expressing chimeric repressor (SRDX) fused to TCP13 cDNA using the TCP13 promoter. TCP13 is a drought-inducible gene and play an essential role in leaf morphology. Arabidopsis plants were grown on the Murashige and Skoog (MS) medium (Murashige and Skoog, 1962), supplemented with 3% sucrose and 0.8 % agar (under a 16-h-day (60 ± 10 μmol photons m−2 s−1 light intensity) and 8-h-night regime for 2 weeks.

2023-07-07 | E-MTAB-9336 | biostudies-arrayexpress

Gm heat 30 min & 5 h

Project description:control plants vs heat treated plants (30 min & 5 h)

2015-08-12 | E-MTAB-2852 | biostudies-arrayexpress

Transcript profiles of soybean organs exposed to dehydration

Project description:We used the microarray to identify dehydration-responsive genes of soybean organs.

2020-03-03 | E-MTAB-7010 | biostudies-arrayexpress

OsTZF1, a CCCH-Tandem Zinc Finger Protein gene, driven under own promoter produces no pleiotropic effects and confers salt tolerance in rice

Project description:The microarray was done using rice seedling plants grown in soil and treated with 250mM NaCl for two days.

2023-06-07 | E-MTAB-11842 | biostudies-arrayexpress

Identification of down stream genes in heat treated HSF triple mutants (hsfA1a, hsfA1b, and hsfA1d)

Project description:To characterize plant heat shock-responsive genes and to identify identified genes that are regulated by of HSFs underheat shock conditions, transcriptome analysis of rice was conducted using microarray. Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07â1.08) under a 16 h light/8 h dark photoperiod (50 Â± 10 Î¼mol photons mâ2 sâ1) at 22Â°C, and were treated for 30 min at 37Â°C.

2017-02-23 | E-MTAB-4505 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data