Ascorbate, ascorbate oxidase and the acclimation of photosynthesis to high light

ABSTRACT: Ascorbate (vitamin C) is an important antioxidant in leaves with multiple functions in photosynthesis and its levels are tightly regulated by light quality. The majority of work to date has focussed on regulation of ascorbate levels via biosynthesis. However, the pathway of ascorbate degradation, which is localised in the apoplast may additionally play a significant role. The degradative pathway begins with ascorbate oxidation by the enzyme ascorbate oxidase (AO) and hence we have examined the impact of manipulation of ascorbate oxidase activity on the content of ascorbate and the acclimation of photosynthesis to changing light levels. Leaf ascorbate content was highly light dependent in Nicotiana tobaccum being double in high compared with low light conditions. In contrast ascorbate

INSTRUMENT(S): Agilent G2505B scanner

ORGANISM(S): Nicotiana tabacum

SUBMITTER: Pete Hedley 

PROVIDER: E-MTAB-4816 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress