Deciphering the transcriptional response mediated by the redox-sensing system HbpS-SenS-SenR from streptomycetes

ABSTRACT: Comparative analyses of the transcriptomes of the Streptomyces coelicolor A3(2) wild-type and the generated hbpSc-senSc-senRc (hsr) mutant under native and oxidative-stressing conditions allowed to identify differentially expressed genes, whose products may enhance the anti-oxidative defense of the bacterium. To obtain well-grown mycelia, spores of S. coelicolor (WT and hsr mutant) were inoculated in 10 ml R2 medium and grown as standing culture at 30C for 16 h and afterwards on a rotary shaker for 16 h after the addition of 90 ml R2 medium. The cultures were washed four times in minimal medium without supplement. The mycelia were suspended in 50 ml R2 medium and divided in two 25 ml-portions, one of which contained H2O2 (0.15 mM). Cultivation was continued at 30 C on a rotary shaker for t

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Streptomyces coelicolor

SUBMITTER: Anika Winkler 

PROVIDER: E-MTAB-4833 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress