Rapid High-Resolution Functional Mapping of Yeast Genomes by Saturated Transposition

ABSTRACT: The yeast Saccharomyces cerevisiae is a highly powerful model for systems genetics. While the advent of ordered deletion libraries has considerably facilitated yeast screening, it has also narrowed the diversity of screenable variants and precluded the exploration of the non-coding genome. Here, we present a versatile, time- and work-efficient method to functionally explore the yeast genome at an unprecedented throughput and resolution, using saturated transposon mutagenesis coupled to high-throughput sequencing. SAturated Transposon Analysis in Yeast (SATAY) allows the one-step mapping of all genetic loci in which a transposon can be inserted without disrupting a function necessary for cell growth. SATAY is especially suited to discover loci important for growth in various conditions. Her

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Benoit Kornmann 

PROVIDER: E-MTAB-4885 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress