Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

P62/SQSTM1 iCLIP in Human Huh-7 cells


ABSTRACT: These are the results of the iCLIP experiment for p62/SQSTM1 in Human Huh-7 cells treated with DMSO. We used iCLIP method to identify the RNA targets of p62 and nucleotide positions of the p62 interaction on RNA. We used 2 replicates and 2 different antibodies against endogenous p62 to enrich protein/RNA complexes. cDNAs were tagged with iCLIP composite barcodes (e.g. NNNTTGTNN) which contain 4 sample-encoding bases (e.g. TTGT) and and 5 random bases (noted with N in NNNTTGTNN example) which serve as unique molecular identifiers to post-filter PCR duplicates. These composite barcodes are found in the read headers (after last colon ':' character) of submitted fastq files.

ORGANISM(S): Homo sapiens

SUBMITTER: Charles Girardot 

PROVIDER: E-MTAB-4894 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2021-07-24 | E-MTAB-9078 | biostudies-arrayexpress
2015-11-09 | E-MTAB-4010 | biostudies-arrayexpress
2016-09-05 | E-GEOD-79178 | biostudies-arrayexpress
2015-05-05 | E-MTAB-3534 | biostudies-arrayexpress
2015-12-10 | E-GEOD-72744 | biostudies-arrayexpress
2013-12-02 | E-GEOD-46488 | biostudies-arrayexpress
| PRJEB19002 | ENA
2010-11-07 | E-GEOD-24622 | biostudies-arrayexpress
2022-07-19 | PXD034901 | Pride
2012-09-21 | E-GEOD-41027 | biostudies-arrayexpress