Effect of estradiol (E2) exposure and KMT2B knockdown on gene expression in MCF-7 cells

ABSTRACT: MCF-7 is an estrogen receptor-positive breast cancer cell line. This experiment is designed to study (1) the effect of estradiol (E2) exposure and (2) lysine methyltransferase 2B (KMT2B) knockdown in MCF-7 cells. Cells were grown for 72 hours prior to treatment with vehicle or 10 nM E2 for 4 and 24 hours. Additionally, to assess the effect of KMT2B knockdown, MCF-7 cells were transfected with KMT2B targeting siRNA or scrambled control siRNA in the absence or presence of E2. RNA were isolated using Trizol and hybridized to Affymetrix GeneChip Human Genome U133 Plus 2.0 array.

INSTRUMENT(S): GeneChip Scanner 3000

ORGANISM(S): Homo sapiens

SUBMITTER: Chia-Hsin Su 

PROVIDER: E-MTAB-4923 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress