Human SU-DHL-5 and TMD-8 B-lymphome cell line subclones

ABSTRACT: Genetic heterogeneity though common in tumors has been rarely documented in cell lines. To examine how often B-lymphoma cell lines are comprised of subclones, we performed immunoglobulin (Ig) heavy chain hypermutation analysis. Revealing that subclones are not rare in B-cell lymphoma cell lines, 6/49 Ig hypermutated cell lines (12%) consisted of subclones with individual Ig mutations. Subclones were also identified in 2/284 leukemia/lymphoma cell lines exhibiting bimodal CD marker expression. We successfully isolated 10 subclones from four cell lines HG3, SU-DHL-5, TMD-8, U-2932). Whole exome sequencing was performed to molecularly characterize these subclones. We in detail describe the clonal structure of cell line HG3, derived from chronic lymphocytic leukemia. HG3 consists of three line

INSTRUMENT(S): Illumina HiSeq 2500, Covaris S2

ORGANISM(S): Homo sapiens

SUBMITTER: Claudia Pommerenke 

PROVIDER: E-MTAB-4956 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress