Microenvironment-driven resistance to B-Raf inhibition in a melanoma patient is accompanied by broad changes of gene methylation and expression in distal fibroblasts. Transcriptomic study.

ABSTRACT: In this study, we compared gene expression and genome methylation of diverse fibroblast populations from a patient suffering from acrolentiginous melanoma (Breslow 4.0 mm, Clark IV, B-Raf V600E mutated). Stromal cells from the metastasis, i.e., melanoma associated fibroblasts (MAF), were positive for smooth muscle actin (SMA). Autologous control fibroblasts (ACF) isolated from distant uninvolved skin of the same patient during B-Raf inhibitor therapy and before clinical progression of the disease exhibited also strong SMA expression. Similar phenotype was observed in control dermal fibroblasts (CDF) from different donors yet exclusively after stimulation by TGF-β1. The identified differences in gene transcription as well as in DNA methylation indicate systemic activation of dermal fibrobl

ORGANISM(S): Homo sapiens

SUBMITTER: Michal Kolar 

PROVIDER: E-MTAB-4964 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress