High throughput sequenceing of fission yeast to survey the dynamic repertoire of a eukaryotic transcriptome at the single nucleotide resolution

ABSTRACT: Recent data from several organisms indicate that the transcribed portions of genomes are larger and more complex than expected, and many functional properties of transcripts are not based on coding sequences but on regulatory sequences in untranslated regions or non-coding RNAs. Alternative start and polyadenylation sites and regulation of intron splicing add additional dimensions to the rich transcriptional output. This transcriptional complexity has been sampled mainly using hybridization-based methods under one or a few conditions. We applied direct high-throughput sequencing of cDNAs, complemented with different expression data from high-density tiling arrays, to globally sample transcripts of the fission yeast Schizosaccharomyces pombe, independently from available gene annotations

INSTRUMENT(S): Illumina Genome Analyzer

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Brian Wilhelm 

PROVIDER: E-MTAB-5 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress